Senna Leaf Extract20%

Short Description:

Senna is a natural herb whose leaves come from the Cassia senna plant. Senna leaves are often sold as part of detox teas because they have a powerful laxative effect and can help relieve constipation, which affects 16 to 33 percent of adults.


Product Detail

Product Tags

Quality Standand

Coa

Product Name: Gentian extract

Latin name:Gentianae Scabrae Radix

Quality standard: National Standard

Specification: 10:1  5%  10%  98%

Test method:HPLC

CAS NO: 20831-76-9

Appearance: Light brown powder

Storage method: Store in cool and dry place. Keep away from strong light and heat.

Shelf life: 24 month

Source of extraction: North gentian root

Package specification: 1kg / aluminum foil bag, 25kg/Drum or as required by customers

Product introduction: Soluble in water

Pictures 

Senna-Leaf-Extract


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  • Determination of sennoside

     1. Instruments and reagents

     

    1.1 high performance liquid chromatography: Shimadzu lc-16 high performance liquid chromatography; equipped with spd-16 UV detector

     

    1.2 ultrasonic cleaning instrument: power 250W, frequency 40KHz

     

    1.3 analytical balance: sensitivity 0.00001g

     

    1.4 volumetric flask (25ml, 10ml)

     

    1.5 pipette (5ml)

     

    1.6 sennoside B reference substance (99% purity)

     

    1.7 sennoside a reference substance (purity 98%)

     

    1.8 acetonitrile (Chromatographic grade)

     

    1.9 phosphoric acid (analytical grade)

     

    1.10 sodium bicarbonate

     

    2. Experimental steps

     

    2.1. Solution preparation

     

    2.1.1 preparation of reference solution

     

    Take an appropriate amount of sennoside A and sennoside B, dry them under reduced pressure for 12 hours, put them into a brown measuring flask, add 0.1% sodium bicarbonate solution to make a mixed solution containing 0.05 mg sennoside A and 0.1 mg sennoside B per 1 ml, and shake well to obtain the reference solution.

     

    2.1.2 preparation of sample solution

     

    The sample is about 0.05 g, accurately weighed, put into a conical flask with a stopper, add an appropriate amount of 0.1% sodium bicarbonate solution, ultrasonic treatment for 15 minutes (30 ~ 40 ), cool, use 0.1% sodium bicarbonate solution to fix the volume, shake well, filter, and take the filtrate to obtain the sample solution.

     

    2.2. Determination

     

    2.2.1 chromatographic conditions

     

    Chromatographic column: wondasil c18-wr 5um 4.6x250mm

     

    Column temperature: 30

     

    Flow rate: 1.0 L / min;

     

    Wavelength: 340nm

     

    Injection volume: 20 μ L;

     

    Mobile phase:

     

    A: Acetonitrile

     

    B: 0.1% phosphoric acid aqueous solution

     

    Gradient condition:

     

    Time [min] %A %B

     

    0 15 85

     

    15 25 75

     

    25 30 70

     

    30 80 20

      

    2.2.2 testing

     

    Under the above chromatographic conditions, after the instrument is stable and the baseline is stable, extract 20 μ l of the control solution and the test solution respectively, inject them into the liquid chromatograph for determination.

     

    5. Content calculation

     

    The content was calculated by external standard method.

     

    Sennoside content (%) = sennoside B + sennoside a

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