This product is made from the whole herb of Centella asiatica (L.) urb.
Properties: the product is light yellow to light brown yellow powder, odorless, bitter and slightly moisture inducing.
This product is soluble in water and ethanol, but insoluble in trichloromethane and ethyl alcohol.
(identification] (1) take about 2mg of this product, put it in a test tube, add 1ml of acetic anhydride, shake well, slowly add 1ml of sulfuric acid along the wall of the test tube, and show purplish red ring at the junction of the two solutions.
(2) Take the powder of this product and add ethanol to make a solution containing 10mg per 1ml as the test solution. In addition, the reference substance of Centella asiatica and Centella asiatica were added with ethanol to prepare a solution containing 10mg each in 1ml as the reference substance solution. According to the test of thin layer chromatography (general rule 0502), absorb the above three solutions of 5 μ 1 each, respectively point them on the same silica gel G thin layer plate, take the upper solution of n-butanol-ethyl acetate-water (4:1:5) as the developing agent, develop, take out, dry the gap, spray acetic anhydride-sulfuric acid-water-free ethanol (1:10) solution, heat at 105 “C until the spots are clear. In the chromatogram of the test sample, there were two blue brown spots corresponding to the chromatogram of the reference sample.
(check) weight loss on drying: take this product and dry it to constant weight at 105 “C. the weight loss shall not exceed 10.0% (general rule 0831).
Determination of heavy metals and harmful elements (general rule)
2321), lead should not exceed 5mg / kg, pickaxe should not exceed 0. 3mg / kg, copper should not exceed 2mg / kg, mining should not exceed 0. 2mg / kg, copper should not exceed 20mg / kg
[fingerprint] was determined by high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system applicability test: octadecyl silica bonded silica gel was used as the filler (column length: 25cm, inner diameter: 4.6mm, particle size: 5 μ m); ethanediol-2mmol / L betacyclodextrin solution (24i76) was used as the mobile phase, and the detection wavelength was 205nm. The number of theoretical plates should not be less than 4000
Preparation of the reference solution: take the reference substance of asiaticoxil and asiaticoxil, weigh them precisely, add methanol to make the solution containing 0.2mg per 1ml.
The preparation of the test solution is the same as the preparation of the test solution under (content determination).
The reference solution and the test solution were precisely absorbed
1, Inject into the liquid chromatograph, measure, record the chromatogram.
According to the similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine, the similarity between the fingerprints of test samples and the control fingerprints should not be less than 0.90
The test solution and the above reference solution under item J of content determination were respectively placed on the same silica gel G thin layer plate with n-butanol-acetic acid hydrochloric acid (8 T 1 I 3) as the control
Peak 2: Jingji snow grass Xifeng 3, snow grass Xifeng 3
The slope sensitivity of the integral parameter is 1, the peak width is o.1, and the minimum peak area is 0.20, and the minimum peak height is 0. S
The content was determined by HPLC (general rule 0512).
The chromatographic conditions and system applicability were tested with octadecyl silica bonded silica gel as filler 1, ethanediol-2mmol / L betacyclodextrin solution (24:76) as mobile phase, and the detection wavelength was 205nm. The number of theoretical plates should not be less than 4000
Preparation of reference solution appropriate amount of Jingji Centella asiatica standard and Centella asiatica standard were precisely weighed, and methanol was added to prepare a solution containing 0.2mg per 1ml.
Preparation of the test solution: take about 50mg of this product, weigh it accurately, and place it in the oven
In a 50ml volumetric flask, add methanol to dissolve, dilute to the scale, shake well, filter, and take the filtrate.
Methods: the control solution and the test solution were precisely aspirated
10 μ 1, injected into the liquid chromatograph for determination.
This product is calculated as dry product, and contains total cyhaloxime (C,. H, a 020)
And Centella asiatica C C48 h1s019) should not be less than 55.0%.
Post time: Apr-25-2021