High Quality (DIM) Diindolylmethane 98%
Product name: Diindolylmethane
Quality standard: National standard
CAS NO: 1968-05-4
Appearance: off-white powder
Storage method: The product should be sealed and stored in a cool and dry place.
Shelf life: 24 months
Source of extraction: Synthetic
Package specification: 1kg / aluminum foil bag, 25kg/Drum or as required by customers.
3,3'-Diindolylmethane (DIM) is a component of Indole-3-carbinol (I3C) found in members of the Brassica family. Most notably broccoli, kale, and cauliflower.
- RangeThis technical requirement specifies the technical requirements, inspection methods, inspection rules and labels of 3,3′-Diindolylmethane, a raw material for health food.This standard is applicable to 3,3′-DiindolylmethaneStandardization of reference documentThe terms in the following documents become terms of this technical requirement by reference to them. All subsequent amendments (excluding errata) or revisions to dated references shall not apply to this Technical Requirement; however, the parties to an agreement under this Technical Requirement are encouraged to study the availability of the latest version of these documents. For undated references, the latest version is applicable to this technical requirement.
- GB/T 5009.11 Determination of total arsenic and inorganic arsenic in foodGB/T 5009.12 Determination of lead in foodsGB/T 5009.15 Determination of cadmium in foods
GB/T 5009.17 Determination of total and organic mercury in foodstuffs
GB/T 5009.19 Determination of multi – component residues of organochlorine pesticides in food
GB 4789.2 Microbiological testing of food hygiene – determination of total number of colonies
GB 4789.3 Microbiological testing of food hygiene – determination of coliform bacteria
GB 4789.15 Food hygiene microbiology test for mold and yeast counts
Pharmacopoeia of the People’s Republic of China (2015 edition)
- Structual formula
3,3′-Diindolylmethane Molecular formula C17H14N2 Molecular weight 246.31 structural formula as followings:.Chart 1 Sensory index
DIM special smell, bitter taste
4.3 Symbolic ingredient
Conform to chart 2 requirement
Chart 2 Symbolic ingredient
According to dry powder DIM purity（g/100g）
More than 30.0
4.4 Physicochemical requirement
Conform to chart 3 requirement
Chart 3 Physicochemical requirement
|Partical size[80 mesh pass （%）] ≧||
|Ash content（%） ≦||
|Hexachloro-cyclohexane soprocide （mg/kg） ≦||
|DDT （mg/kg） ≦||
|Huintozene （mg/kg） ≦||
4.5 Microbiological indicator
Conform to chart 4 requirement
Chart 4 Microbiological indicators
|Total Plate Count/（cfu/g） ≦||
|Yeast & Mold/（cfu/g） ≦||
5 Test method
5.1 After opening the sample, smell its smell and taste its taste immediately; In addition, appropriate amount of the sample was placed in a white porcelain plate to observe its color and appearance, and to check for foreign bodies.
5.2 Physicochemical requirement
5.2.1 The purity of 3,3′-Diindolylmethane
The method was determined according to Appendix A
The test was carried out according to the screening method stipulated in the General Rule 0982(Second Method) of the Fourth Part of the Pharmacopoeia of the People’s Republic of China (2015).
The assay was conducted according to the method specified in General Rule 0832(second method) of the fourth part of Pharmacopoeia of the People’s Republic of China (2015 edition).
5.2.4 Ash content
The assay was carried out according to the method specified in General Rule 1106 of the fourth part of Pharmacopoeia of the People’s Republic of China (2015 edition)
The total arsenic and inorganic arsenic in food were determined according to GB/T 5009.11 method.
According to GB/T 5009.12 for the determination of lead in food.
Cadmium in food was determined according to GB/T 5009.15.
The total mercury and inorganic mercury in food were determined according to GB/T 5009.17.
5.2.9 Hexachloro-cyclohexane soprocide DDT Huintozene
Execute according to GB/T 5009.19
5.4 Bacteriological examination
5.4.1 Total Plate Count
In accordance with the “national food hygiene standard” GB4789.2
5.4.2 Yeast & Mold
In accordance with the “national food hygiene standard” GB4789.15
In accordance with the “national food hygiene standard” GB4789.3
In accordance with the “national food hygiene standard” GB4789.4
In accordance with the “national food hygiene standard” GB4789.10
6 Test rule
Samples were taken according to the method specified in the General Rule 0211 of the Four Parts of the Pharmacopoeia of the People’s Republic of China (2015 edition), and the products that were evenly mixed and had the same nature and quality within a certain limit were tested as the same batch.
6.2 Inspection Classification
6.2.1 Delivery inspection
Before leaving the factory, the quality inspection department of the factory shall inspect the products batch by batch according to the requirements of 4.2 ~ 4.5, and the products that pass the inspection and issue the quality certificate can be sold before leaving the factory.
6.2.2 Test type
Carry out type inspection according to chapter 4.Type inspection shall be carried out every six months in case of any of the following situations:
A) when there is a big change in raw materials;
B) Adjustment of key processes;
C) Resuming production after replacing equipment or stopping production;
D) There is a big difference between the results of ex-factory inspection and the last type inspection.
6.3 Decision rules
6.3.1 If one of the inspection results does not meet the technical requirements, samples of twice the quantity shall be randomly selected from the same batch of products for re-inspection, and the re-inspection result shall prevail. If there is still one index unqualified in the reinspection, the batch of products will be judged as unqualified.
6.3.2 If any item in the microbial project does not meet the technical requirements, it will be judged as unqualified and will not be re-inspected.
6.3.3 Determination of type inspection is the same as that of factory inspection.
Labeling, packaging, transportation and storage
7.1 The label
7.1.1 The packaging label shall mark: sample name, date of production, batch number of production, specification, gross weight, net weight and execution standard.
7.1.2 The label content is clearly visible and the label is firmly pasted.
7.2 The packing
Packaging materials should meet the requirements of food hygiene.The packaging materials shall be inspected strictly before use.After the barrel is packed, it should be sealed with a seal label.
7.3.1 The means of transport shall be clean and hygienic, and shall not be mixed with poisonous, harmful, corrosive or odor articles.
7.3.2 Light load and light discharge shall be carried out, and extrusion, exposure and rain shall be prevented during transportation.
7.4.1 The product shall not be stored mixed with toxic, harmful, corrosive or peculiar smell.
7.4.2 The product should be sealed and stored in a cool and dry place.
7.5 Shelf life
Shelf life is 24 months.
Determination of diindolylmethane
1. Objective to establish the standard operating procedure for the determination of diindolylmethane by HPLC.
2. SCOPE: suitable for the determination of diindolylmethane.
3. Reference: diindolylmethane. The above standard is provided by Sigma-aldrich.
4.1 methanol (chromatographic purity)
4.2 methanol (analytical purity)
4.3 water (ultrapure water)
5.1 High-performance Liquid Chromatography; UV DETECTOR
5.2 Analytical Balance: sensitivity 0.00001G
5.3 ULTRASONIC CLEANING INSTRUMENT: Power 250W, frequency 40KHz
5.4 glass flow phase filter (0.45 m)5.5 needle microporous filter membrane (0.45 m)5.6 glass volumetric flask (50 ml) , measuring cylinder (100 ML) , etc. .
6. Preparation of solution:
6.1 preparation of Reference Solution: precision weighing to dry to constant weight of diindolylmethane reference substance, add methanol to make 1 ML of solution containing 0.02 mg, respectively, as the reference solution.
6.2 preparation of the sample solution: the sample is precisely weighed and measured in a 50ML flask, the methanol is added in about 40ml, after 30 minutes of ultrasonic treatment, it is taken out at room temperature, diluted with methanol to the scale, shaken, and filtered by 0.45 m filter membrane. Kill.
7.CHROMATOGRAPHIC CONDITIONS: CHROMATOGRAPHIC COLUMN: Agilent TC-C18(250 mm 4.6 mm, 5 m) detection wavelength: 280 NM
flow rate: 1.0 ML/MIN INJECTION VOLUME: 10 L COLUMN TEMPERATURE: 30 °C mobile phase:
A: ACETONITRILE B: water
system adaptability: theoretical plate number not less than 3000
8 in terms of diindolylmethane.
SAMPLE DETERMINATION: under the above chromatographic conditions, after the stable base line of the instrument is stable, 10 l of the control solution and 10 l of the sample solution are precisely absorbed, and the diindolylmethane is determined for 12 Min, respectively.
9. Content calculation:
Where a 1: Peak area of diindolylmethane in sample solution a 0: Peak area of diindolylmethane in control solution
w 1: SAMPLE WEIGHT MG W 0: Sample Weight MG v 1: Sample Volume ML v 0: sample volume ML K: purity of control, sample content results were taken as the mean values of two parallel samples.